The Proteolytic Enzyme Test for Detecting Incomplete Antibodies.

نویسندگان

J A Morton

M M Pickles

چکیده

The specific agglutination by incomplete anti D antibodies of a saline suspension of red cells, after preliminary incubation with a solution of crystalline trypsin, has been previously reported (Morton and Pickles, 1947). During the past two years this method has been standardized and used as a test for the detection of incomplete antibodies. The trypsin-treated cells have been found to give reliable results and to be more sensitive than the concentrated albumin (Diamond and Denton, 1945) and anti-globulin (Coombs, Mourant, and Race, 1945) methods in the detection of weak incomplete anti D antibodies. The utilization of trypsin-treated red cells for the routine detection of anti Rh antibodies has been reported by Wheeler, Luhby, and Scholl (1950) and also in the studies of haemagglutinins in congenital and acquired haemolytic anaemias by Wright, Dodd, and Bouroncle (1949). In view of the high cost of the crystalline trypsin previously used from the Plaut Research Laboratory, New Jersey, and of the unreliability of some of the commercial varieties of trypsin which have been tested, a search has been made for other proteolytic enzymes which would react in the same manner. This paper reports an investigation of proteolytic enzymes which could be used as a substitute for crystalline trypsin, the standardization of the test, and its reliability and specificity as a result of two years' experience in routine antibody determinations.

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